Datasheet

Bacteroides fragilis; B56-3

Bacteroides fragilis was isolated from sewage water. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

Product Information
Product Overview	Bacteroides fragilis was isolated from sewage water. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
Target	Bacteroides
Genus	Bacteroides
Strain Designation	B56-3
Application	Detection of bacteriophages
Culture Conditions	37°C; Anaerobic
Source	Sewage water, 1996
Risk Group	1
Product Format	Freeze-dried
Packaging	Ampoule tube
Storage	-80°C
Shelf Life	6 years

Target Introduction
Introduction	Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names	Bacteroides fragilis; B56-3

Target Introduction

Introduction

Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.

Alternative Names

Bacteroides fragilis; B56-3

FAQs Published Data Customer Reviews Related Products

How should Bacteroides fragilis B56-3 be stored?
For long-term storage, Bacteroides fragilis B56-3 should be kept freeze-dried at -80°C. This method ensures the preservation of the bacterial strain's viability and genetic stability over extended periods.
Can Bacteroides fragilis B56-3 be used in clinical settings?
Currently, Bacteroides fragilis B56-3 is designated for research use only. It is not intended for use in clinical diagnostics or therapeutic procedures. Researchers should ensure compliance with relevant guidelines and regulations when handling this strain.
What are the potential research applications of Bacteroides fragilis B56-3?
Bacteroides fragilis B56-3 serves as a valuable tool in microbiological research, particularly in the study of host-microbe interactions and the modulation of gut microbiota. It is also used in the exploration of bacteriophage biology, investigating the phages that infect B. fragilis as potential therapeutic agents.
How is Bacteroides fragilis B56-3 prepared for experimental use?
Bacteroides fragilis B56-3 is provided in a freeze-dried format and should be carefully rehydrated according to the instructions provided with the product. It is important to handle the rehydration fluid under anaerobic conditions to prevent exposure to oxygen, which could harm the bacteria.
How can the viability of Bacteroides fragilis B56-3 be confirmed post-revival?
Post-revival viability can be confirmed by culturing Bacteroides fragilis B56-3 on suitable anaerobic media and checking for typical colony morphology. Growth should be monitored closely to ensure that the bacteria are active and healthy.

Bacteroides-fragilis-B56-3-1

Duplex PCR

The research discusses the use of Bacteroides fragilis strain B56-3 for the simultaneous detection of feces-specific bacteriophages in water bodies. The development of a novel duplex PCR assay is outlined, capable of identifying bacteriophages that infect Bacteroides fragilis strains, which serve as indicators of human-specific and general fecal contamination in environmental samples. This method marks a significant improvement over traditional anaerobic cultivation techniques, which are more time-consuming, requiring 1-2 days of incubation. The duplex PCR assay developed in this study can detect these bacteriophages within a few hours, thereby expediting water quality monitoring.
The assay demonstrated high sensitivity and specificity, reducing potential false positives associated with other bacteriophages or bacterial hosts. The document illustrates that the PCR method could detect very low concentrations of phages, down to 10^-5 ng of DNA, highlighting its efficiency. Such advancements are crucial for rapid assessment and management of water quality, particularly in settings where quick results are essential to prevent public health risks from fecal contamination.

Chyerochana, N., Javed, B. P., et al. Simultaneous detection of feces-specific bacteriophages of Bacteroides fragilis with a duplex PCR assay. Environment and Natural Resources Journal. 2018, 16(1): 82-90.

High-Quality Strain
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Using Bacteroides fragilis; B56-3 has streamlined our research process, thanks to its high adaptability and growth efficiency, making it a preferred choice over others.
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Creative Biolabs not only provides an excellent Bacteroides fragilis; B56-3 product but also backs it with expert support, enhancing the value of our research investment.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.